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تولید طعم با استفاده از مخمر توسط تخمیر جامد پسماند های پوست پرتقال

عنوان انگلیسی مقاله:

Yeast flavour production by solid state fermentation of orange peel waste

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3. Results and discussion

3.1. Growth characteristics of S. cerevisiae during SSF of autoclave sterile and non-sterile OP

The main limitation of OP usage as a fermentation feedstock is the presence of d-limonene found in the peel oil at a percentage higher than 90% (w/w). The strong inhibitory effect of this compound towards yeast growth has been attributed to disruption of cellular membrane and H+ and K+ transport energized by glycolysis [29]. Thus, protecting the yeast against this risk factor helps in preventing failure of the fermentation process. In view of the above, in the first series of experiments, evaluation of the necessity of autoclave treatment of OP for the elimination of yeast-inhibiting volatile substances, mainly d-limonene, and natural microflora present in this material took place. To achieve this goal preliminary experiment involved SSF of non-sterile and autoclave sterile OP inoculated with Vitilevure MT. The kinetic data obtained are presented in Fig. 1.