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دانلود رایکان مقاله انگلیسی ISI با موضوع بارکدینگ DNA ماده گیاهی دارویی برای شناسایی

عنوان فارسی مقاله:

بارکدینگ DNA ماده گیاهی دارویی برای شناسایی

عنوان انگلیسی مقاله:

DNA barcoding of medicinal plant material for identification

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Introduction
The traditional system of medicine utilizes medicinal plants to cure various ailments but the herbal industry suffers from substitution and adulteration of medicinal herbs with closely related species. The efficacy of the drug decreases if it is adulterated, and in some cases, can be lethal if it is substituted with toxic adulterants. Hence, the correct formulation is important for the medicinal herb to be effective. The traditional methods of medicinal plant identification include organoleptic methods (identification by the senses: taste, sight, smell, touch), macroscopic and microscopic methods (identification by shape, colour, texture) and chemical profiling (e.g. TLC, HPLC-UV, HPLC-MS). However, neither method can identify the related species easily in processed products because the former method requires trained personal for macroscopic and microscopic examinations. In the latter method, chemical profiles or markers may be affected by physiological and storage conditions. Authentication at the DNA level provides more reliability because, in contrast to RNA, DNA is a stable macromolecule that is not affected by external factors and is found in all tissues. Therefore, development of DNA-based markers is important for authentication of medicinal plants.

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