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دانلود رایکان مقاله انگلیسی ISI با موضوع دی ان ای شناخته شده Dientamoeba fragilis روی سطح تخم های نابارور انتروبیوس ورمیکولاریس

عنوان فارسی مقاله:

دی ان ای شناخته شده Dientamoeba fragilis روی سطح تخم های نابارور انتروبیوس ورمیکولاریس

عنوان انگلیسی مقاله:

DNA of Dientamoeba fragilis detected within surface-sterilized eggs of Enterobius vermicularis

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4. Discussion

The present study is, to our knowledge, the first to demonstrate the presence of D. fragilis DNA inside eggs of E. vermicularis, allowing us to presume that D. fragilis can be transmitted by a vector, in this case via eggs of E. vermicularis. The rationale behind this presumption is the repeated detection of D. fragilis DNA from pinworm eggs, which had been surface-sterilized prior to DNA extraction using a solution of hypochlorite in concentrations shown to render DNA non-amplifiable due to DNA damage. In addition, since water from the final washing steps was PCR-negative for both E. vermicularis and D. fragilis, we conclude that D. fragilis DNA amplified from DNA extracts from the surface-sterilised eggs must have been present inside the pinworm eggs and not on the surface. It is possible that intact D. fragilis trophozoites (or an undetected cyst stage) can stick to the surface of an egg, as suggested by Menghi et al. (2005), who noted amoeboid-like structures on the surface of the eggs. However, we consider this unlikely, given that D. fragilis normally degrades rapidly (<48 h) once passed from the intestine, and since the mean time from sampling to processing of the cellophane tape samples was 36 days. Also, any organism adherent to the surface of the egg would still be subjected to the damaging effect of the hypochlorite