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دانلود رایکان مقاله انگلیسی ISI با موضوع بیان متفاوت mRNA های کانال سدیمی در بافت های عصب دار

عنوان فارسی مقاله:

بیان متفاوت mRNA های کانال سدیمی در بافت های عصب دار و سیستم عصبی محیطی موش (rat)

عنوان انگلیسی مقاله:

Differential expression of sodium channel mRNAs in rat peripheral nervous system and innervated tissues

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بخشی از مقاله انگلیسی :

3. RESULTS AND DISCUSSION

Total RNA was extracted from a variety of rat tissues at early and late postnatal stages of development and was subjected to RNA blot hybridization analysis using RNA probes [24] specific for sodium channel I, II and III mRNAs or a coding region probe (common probe) that recognizes the mRNAs of these three sodium channels. Results obtained with the specific probes in the different regions, along with a few CNS regions for comparison, are shown in figs 1 and 2. Hybridization signals detected in the PNS with the common probe are compared to brain signals in fig.3. The temporal patterns of mRNA expression in total brain and spinal cord, described earlier [6], could be recognized even when only one early and one late postnatal stage of development was analyzed. Therefore, postnatal day 7 (P7; P15 optic nerve is an exception) and late postnatal stages P30, P60 or P90 have been selected as reference days to monitor changes during development. In order to directly compare the relative mRNA levels of the various regions at the two different time points (fig. l), the RNAs of all these tissues were contained in the same blots and all were hybridized simultaneously.